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Scheme of surface tethered ribosomes synthesizing GFP and the employed setup for simultaneous dual color imaging.

Protein synthesis and co-translational folding
Protein synthesis is a fundamental cellular process, by which ribosomes decode genetic information and convert it into an amino acid sequence. It is accomplished by the translational machinery (ribosomes), accessory proteins, tRNA , mRNA and various factors. Synthesis and translation does not necessarily require cell integrity, but can also take place as so-called cell-free synthesis. This opens the door for comprehensive studies to obtain a deeper understanding of individual steps of the translation cycle and of the folding of de novo synthesized proteins. We have monitored the biosynthesis of the green fluorescence protein on single-molecule level in real time. Because co-translational folding is of particular importance for multi-domain proteins, we are currently developing approaches to monitor this kind of folding with PGK constructs.

Biophysics (Aachen):
Jörg Fitter

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