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FRET: Bends and Kinks in the DNA

Bends and kinks in the DNA
We present advances in the use of single-molecule FRET measurements with flexibly linked dyes to derive full 3D structures of DNA constructs based on absolute distances. The resolution obtained by this single-molecule approach harbours the potential to study in detail also protein- or damage-induced DNA bending. If one is to generate a geometric structural model, distances between fixed positions are needed. These are usually not experimentally accessible because of unknown fluorophore-linker mobility effects that lead to a distribution of FRET efficiencies and distances. To solve this problem, we performed studies on DNA double-helices by systematically varying donor acceptor distances from 2 to 10 nm.

Molecular physical chemistry:
Anna K. Wozniak, Filipp Oesterhelt, Claus Seidel

Copyright: Heinrich Heine University Düsseldorf