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of Biophysics and Soft Matter

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Standard Techniques in Biochemistry and Biophysics

J. Fitter (Biophysics), P. Huesgen (Analytics), W. Hoyer, M. Sevenich, L. Nagel-Steger, G. Schröder (Structural Biochemistry)
scientific coordinators: J. Fitter (Biophysics), B. König (Structural Biochemistry)

In most research projects dealing with biological macromolecules various standard techniques (e.g., absorption-, CD-, or fluorescence spectroscopy, calorimetry, chromatography, mass spectroscopy, centrifugation, or computer based approaches) are employed to characterize or to help for preparing samples. On the one hand, the application of such methods are often a basic prerequisite before more specialized methods (such as x-ray crystallography, NMR, cryo-EM) can be used. On the other hand, results obtained by these basic methods are often of scientific value by themselves. In this lecture the following topics will be presented.

  • Absorption, CD, and basic fluorescence spectroscopy (Jörg Fitter)
    Wednesday, May 8, 1:00pm-2:30pm (building 01.3, room 3001)
  • Mass spectrometry and proteomics (Pitter Huesgen)
    Wednesday, May 22, 1:00pm-2:30pm (building 01.3, room 3001)
  • Inter-molecular binding and interaction: isothermal calorimetry and surface plasmon resonance spectroscopy (Wolfgang Hoyer and Marc Sevenich)
    Wednesday, June 5, 1:00pm-2:30pm (building 15.9, room 4001)
  • Chromatography, electrophoresis, and ultracentrifugation (Luitgard Nagel-Steger)
    Wednesday, June 19, 1:00pm-2:30pm (building 15.1, room 249)
  • Bioinformatic tools: sequence analysis, homology modeling, display modeling of protein structures (Gunnar Schröder)
    Wednesday, July 3, 1:00pm-2:30pm (building 15.1, room 249)

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