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Methodology and Lab Equipment

Students in our laboratory can expect to gain experience in several interesting and modern techniques and methods, including:

  • Molecular microbiology: Cloning techniques, site-directed mutagenesis, Gibson assembly
  • Phage biology: Isolation, sequencing, assembly and annotation, infection studies
  • Single-cell analysis: time-lapse fluorescence microscopy (Live Cell Imaging), flow cytometry and cell sorting (FACS)
  • OMICS techniques: in particular genomics, transcriptomics (microarrays & RNA-Seq), ChIP-Seq, ChAP-Seq (Fig. 5), and proteomics
  • Next-generation sequencing: genome re-sequencing and RNA-Seq (Illumina MiSeq, Nanopore)
  • Bioinformatics & data science: analysis and statistical verification of scientific datasets using Python, R and in-house developed pipelines

Graphic Principle of ChIP-Seq analysisFigure 5: Principle of ChIP-Seq analysis: Cells expressing a regulator are harvested at specific time points. The DNA bound regulator is fixed with formaldehyde prior to cell disruption and DNA shearing. Regulator specific antibodies are linked to proteinA bound to magnetic beads. The regulator can be purified and co-purified DNA is sequenced for the identification of genomic targets. Modified from Keppel et al., 2020, doi: 10.1093/nar/gkaa415.